Research Articles

  1. Erin W Kavanagh , Stephany Y Tzeng , Neeraj Sharma , Garry R Cutting, Jordan J Green. Ligand-free biodegradable poly(beta-amino ester) nanoparticles for targeted systemic delivery of mRNA to the lungs. Biomaterials. 2025 Feb:313:122753.
  2. Audrey Pion, Erin Kavanagh, Anya T. Joynt, Karen S. Raraigh, Lori Vanscoy, Elinor Langfelder-Schwind, John McNamara, Brooke Moore, Shivani Patel, Kate Merlo, Renee Temme, Valeria Capurro, Emanuela Pesce, Christian Merlo, Nicoletta Pedemonte, Garry Cutting, Neeraj Sharma*. Investigation of CFTR function in human nasal epithelial cells informs personalized medicine. Am J Respir Cell Mol Biol. 2024 Nov;71(5):577-588.
  3. Alice C. Eastman, Gedge Rosson , Noori Kim, Sewon Kang, Karen Raraigh , Loyal A. Goff, Christian Merlo, Noah Lechtzin, Garry R. Cutting, Neeraj Sharma*. Eccrine sweat gland characterization and establishment of a primary culture technique for ion transport evaluation. J Cyst Fibros. 2024 Jul 4:S1569-1993(24)00783-5.
  4. Karen Mention , Kader Cavusoglu-Doran , Anya T Joynt, Lúcia Santos , David Sanz , Alice C Eastman, Christian Merlo , Elinor Langfelder-Schwind , Martina F Scallan , Carlos M Farinha , Garry R Cutting , Neeraj Sharma , Patrick T Harrison . Use of adenine base editing and homology-independent targeted integration strategies to correct the cystic fibrosis causing variant, W1282X. Human Molecular Genetics. 2023 Nov 17;32(23):3237-3248.
  5. Anya T. Joynt, Erin W. Kavanagh, Gregory A. Newby, Shakela Mitchell, Alice C. Eastman, Kathleen C. Paul, Alyssa D. Bowling, Derek L. Osorio, Christian A. Merlo, Shivani U. Patel, Karen S. Raraigh, David R. Liu, Neeraj Sharma, Garry R. Cutting. Protospacer modification improves base editing of a canonical splice site variant and recovery of CFTR function in human airway epithelial cells. Molecular Therapy – Nucleic Acids. 2023, 33, 335-350.
  6. Kwak G, Gololobova O, Sharma N, Caine C, Mazur M, Mulka K, West NE, Solomon GM, Cutting GR, Witwer KW, Rowe SM, Paulaitis M, Aslanidi G, Suk JS. Extracellular vesicles enhance pulmonary transduction of stably associated adeno-associated virus following intratracheal administration.J Extracell Vesicles. 2023 Jun;12(6):e12324.
  7. Bowling A, Eastman A, Merlo C, Lin G, West N, Patel S, Cutting G, Sharma N*. Downstream Alternate Start Site Allows N-Terminal Nonsense Variants to Escape NMD and Results in Functional Recovery by Readthrough and Modulator Combination. J Pers Med. 2022 Sep 1;12(9):1448. * Corresponding author
  8. Raraigh KS, Paul KC, Goralski JL, Worthington EN, Faino AV, Sciortino S, Wang Y, Aksit MA, Ling H, Osorio DL, Onchiri FM, Patel SU, Merlo CA, Montemayor K, Gibson RL, West NE, Thakerar A, Bridges RJ, Sheppard DN, Sharma N, Cutting GR. CFTR bearing variant p.Phe312del exhibits function inconsistent with phenotype and negligible response to ivacaftor. JCI Insight. 2022 Mar 22;7(6):e148841.
  9. Gao D, Morini E, Salani M, Krauson AJ, Chekuri A, Sharma N, Ragavendran A, Erdin S, Logan EM, Li W, Dakka A, Narasimhan J, Zhao X, Naryshkin N, Trotta CR, Effenberger KA, Woll MG, Gabbeta V, Karp G, Yu Y, Johnson G, Paquette WD, Cutting GR, Talkowski ME, Slaugenhaupt SA. A deep learning approach to identify gene targets of a therapeutic for human splicing disorders. Nat Commun. 2021 Jun 7;12(1):3332.
  10. Anya T. Joynt, Taylor A. Evans, Matthew J. Pellicore, Emily F. Davis-Marcisak, Melis A. Aksit, Alice C. Eastman, Shivani Patel, Kathleen C. Paul, Derek L. Osorio, Alyssa D. Bowling, Calvin U. Cotton, Karen S. Raraigh, Natalie West, Christian Merlo, Garry R. Cutting, Neeraj Sharma*. Evaluation of both exonic and intronic variants for effects on RNA splicing allows for accurate assessment of the effectiveness of precision therapies. PLoS Genet. 2020 Oct 21;16(10):e1009100. * Corresponding author
  11. Taylor-Cousar JL, Evans TA, Cutting GR, Sharma N*. Potentially lethal cystic fibrosis gene variant in the orangutan. Am J Primatol. 2020;22:e23097.
  12. Aksit MA, Bowling AD, Evans TA, Joynt AT, Osorio D, Patel S, West N, Merlo C, Sosnay PR, Cutting GR, Sharma N*. Decreased mRNA and protein stability of W1282X limits response to modulator therapy. J Cyst Fibros. 2019;18(5):606-613. * Corresponding author
  13. McCague AF, Raraigh KS, Pellicore MJ, Davis-Marcisak EF, Evans TA, Han ST, Lu Z, Joynt AT, Sharma N, Castellani C, Collaco JM, Corey M, Lewis MH, Penland CM, Rommens JM, Stephenson AL, Sosnay PR, Cutting GR. Correlating Cystic Fibrosis Transmembrane Conductance Regulator Function with Clinical Features to Inform Precision Treatment of Cystic Fibrosis. Am J Respir Crit Care Med. 2019;199(9):1116-1126.
  14. *Na CH, *Sharma N, Madugundu AK, Chen R, Aksit MA, Rosson GD, Cutting GR, Pandey A. Integrated Transcriptomic and Proteomic Analysis of Human Eccrine Sweat Glands Identifies Missing and Novel Proteins. Mol Cell Proteomics. 2019;18(7):1382-1395. * Equal contribution first authors.
  15. *Sharma N, Evans TA, Pellicore MJ, Davis E, Aksit MA, McCague AF, Joynt AT, Lu Z, Han ST, Anzmann AF, Lam AN, Thaxton A, West N, Merlo C, Gottschalk LB, Raraigh KS, Sosnay PR, Cotton CU, *Cutting GR. Capitalizing on the heterogeneous effects of CFTR nonsense and frameshift variants to inform therapeutic strategy for cystic fibrosis. PLoS Genet. 2018;14(11):e1007723. * Corresponding authors.
  16. Raraigh KS, Han ST, Davis E, Evans TA, Pellicore MJ, McCague AF, Joynt AT, Lu Z, Atalar M, Sharma N, Sheridan MB, Sosnay PR, Cutting GR. Functional Assays Are Essential for Interpretation of Missense Variants Associated with Variable Expressivity. Am J Hum Genet. 2018; 102(6):1062-1077.
  17. Lee M, Roos P, Sharma N, Atalar M, Evans TA, Pellicore MJ, Davis E, Lam AN, Stanley SE, Khalil SE, Solomon GM, Walker D, Raraigh KS, Vecchio-Pagan B, Armanios M, Cutting GR. Systematic computational identification of variants that activate exonic and intronic cryptic splice sites. Am J Hum Genet. 2017; May 4;100(5):751-765.
  18. Vecchio-Pagán B, Blackman SM, Lee M, Atalar M, Pellicore MJ, Pace RG, Franca AL, Raraigh KS, Sharma N, Knowles MR, Cutting GR. Deep resequencing of CFTR in 762 F508del homozygotes reveals clusters of non-coding variants associated with cystic fibrosis disease traits. 2016. Human genome variation 3 (1), 1-9.
  19. Lee M, Vecchio-Pagán B, Sharma N, Waheed A, Li X, Raraigh KS, Robbins S, Han ST, Franca AL, Pellicore MJ, Evans TA, Arcara KM, Nguyen H, Luan S, Belchis D, Hertecant J, Zabner J, Sly WS, Cutting GR.Loss of carbonic anhydrase XII function in individuals with elevated sweat chloride concentration and pulmonary airway disease. 2016. Human molecular genetics 25 (10), 1923-1933.
  20. Gottschalk LB, Vecchio-Pagan B, Sharma N, Han ST, Franca A, Wohler ES, Batista DA, Goff LA, Cutting GR. Creation and characterization of an airway epithelial cell line for stable expression of CFTR variants. 2016. Journal of Cystic Fibrosis 15 (3), 285-294.
  21. Neeraj Sharma, Jessica LaRusch, Patrick R Sosnay, Laura B Gottschalk, Andrea P Lopez, Matthew J Pellicore, Taylor Evans, Emily Davis, Melis Atalar, Chan-Hyun Na, Gedge D Rosson , Deborah Belchis , Michal Milewski, Akhilesh Pandey , Garry R Cutting. A sequence upstream of canonical PDZ-binding motif within CFTR COOH-terminus enhances NHERF1 interaction. American Journal of Physiology-Lung Cellular and Molecular Physiology. 2016.311(6):L1170-L1182.
  22. J Park, N Sharma, GR Cutting. Melanocortin 3 receptor has a 5′ exon that directs translation of apically localized protein from the second in-frame ATG. 2014. Molecular Endocrinology 28 (9), 1547-1557.
  23. Neeraj Sharma, Patrick R Sosnay, Anabela S Ramalho, Christopher Douville, Arianna Franca, Laura B Gottschalk, Jeenah Park, Melissa Lee, Briana Vecchio-Pagan, Karen S Raraigh, Margarida D Amaral, Rachel Karchin, Garry R Cutting. Experimental assessment of splicing variants using expression minigenes and comparison with in silico predictions. Human mutation. 2014. 35 (10), 1249-1259.
  24. Patrick R Sosnay , Karen R Siklosi, Fredrick Van Goor, Kyle Kaniecki, Neeraj Sharma, Anabela S Ramalho, Margarida D Amaral, Ruslan Dorfman, Julian Zielenski, David L Masica, Rachel Karchin, Linda Millen, Philip J Thomas, George P Patrinos, Mary Corey, Michelle H Lewis, Johanna M Rommens, Carlo Castellani, Christopher M Penland, Garry R Cutting. Defining the disease liability of variants in the cystic fibrosis transmembrane conductance regulator gene. Nature genetics. 2013. 45 (10), 1160-1167.
  25. N Sharma, N Acharya, SK Singh, M Singh, U Sharma, R Prasad Heterogenous spectrum of CFTR gene mutations in Indian patients with congenital absence of vas deferens. 2009. Human reproduction 24 (5), 1229-1236.
  26. N Sharma, M Singh, G Kaur, BR Thapa, R Prasad Identification and characterization of CFTR gene mutations in Indian CF patients. Annals of human genetics. 2009. 73 (1), 26-33.
  27. N Sharma, M Singh, N Acharya, SK Singh, BR Thapa, G Kaur, R Prasad Implication of the cystic fibrosis transmembrane conductance regulator gene in infertile family members of Indian CF patients. 2008. Biochemical genetics 46 (11-12), 847-856.
  28. SK Agrawal, P Kumar, R Rathi, N Sharma, R Das, R Prasad, A Narang UGT1A1 gene polymorphisms in North Indian neonates presenting with unconjugated hyperbilirubinemia. 2009. Pediatric research 65 (6), 675-680.
  29. MS Shahab, P Kumar, N Sharma, A Narang, R Prasad Evaluation of oxidant and antioxidant status in term neonates: a plausible protective role of bilirubin. Molecular and cellular biochemistry. 2008. 317 (2), 51-53.
  30. K Pawan, S Neeraj, K Sandeep, RK Ratho, P Rajendra. Upregulation of Slc39a10 gene expression in response to thyroid hormones in intestine and kidney. Biochimica et Biophysica Acta (BBA)-Gene Structure and Expression. 2007. 1769(2):117-23.
  31. J Kaur, N Sharma, S Attri, L Gogia, R Prasad. Kinetic characterization of Zinc transport process and its inhibition by Cadmium in isolated rat renal basolateral membrane vesicles: In vitro and In vivo studies. Molecular and cellular biochemistry. 2006. 283 (1-2), 169-179.
  32. S Attri, N Sharma, S Jahagirdar, BR Thapa, R Prasad. Erythrocyte metabolism and antioxidant status of patients with Wilson disease with hemolytic anemia. 2006. Pediatric research 59 (4), 593-597.
  33. P.C. Behera, N. Sharma, P.C. Bisoi. Seasonal Variation of Clinically Important Blood Biochemical Constitutents of Lambs. 2005. Indian Vet J. 82, 26-28.
  34. H. Pattnaik, P.C. Bisoi, P.C. Behera, N. Sharma. Effect of Anticoagulants on the Blood Enzymes of the Black Bengal Goat. 2003. Indian Vet. J. 80: 860-863.

Review Articles

  1. Meenu Singh, Cristina Rebordosa, Juliane Bernholz, Neeraj Sharma*. Epidemiology and genetics of cystic fibrosis in Asia: In preparation for the next‐generation treatments. * Corresponding author. Respirology 2015;20(8), 1172-1181.
  2. N Sharma, G Cutting. The genetics and genomics of cystic fibrosis. J Cyst Fibros. 2019; pii: S1569-1993(19)30965-8.
  3. Joynt AT, Cutting GR, Sharma N*. Genetics of Cystic Fibrosis: Clinical Implications. Clin Chest Med. 2022 Dec;43(4):591-602. * Corresponding author

Book Chapters

  1. Meenu Singh, Rashmi Ranjna Das, Neeraj Sharma. Cystic fibrosis. NCCP: Textbook of respiratory medicine. First Edition 2011. Editor: D. Behera. Publisher: JayPee Brothers Medical Publishers.
  2. Neeraj Sharma, Himanshu Sharma, Ujjawal Sharma, Rajendra Prasad. Biochemistry, molecular biology and molecular genetics of cystic fibrosis. Textbook: Biochemistry, molecular biology and molecular genetics of genetic disorders. First Edition 2013. Editor: Rajendra Prasad. Publisher: Transworld Research Network.